2. Wash in water.
3. Differentiate in 2 per cent acetic or dilute hydrochloric acid 1-2 minutes.
4. Wash thoroughly in water.
5. Mount in lævulose or glycerin-gelatin. Amyloid ruby red; tissue blue-violet.
3. Gentian Violet.
Use same method as for methyl violet. The same color-effects are produced.
4. Methyl Green.
Use methyl green in the same way as methyl violet. Amyloid sky-blue or violet; tissue is green.
5. Iodine Green.
1. Stain for 24 hours in a ⅓ per cent water solution of iodine green.