2. Wash in water.
3. Mount in lævulose, glycerin or glycerin-gelatin. Amyloid red violet; tissue green.
6. Birch-Hirschfeld’s Method.
1. Stain in a 2 per cent alcoholic solution of Bismarck brown for 5 minutes.
2. Wash in absolute alcohol.
3. Wash in water.
4. Stain in a 2 per cent water solution of methyl-violet (or a 20 per cent gentian violet) for 5 minutes.
5. Differentiate in 1 per cent acetic acid until the non-amyloid parts are brown.
6. Wash thoroughly in water.
7. Mount in lævulose, glycerin or glycerin-gelatin. Nuclei are brown; amyloid ruby red.