7. Green’s Method.
To a few cc. of hæmalum in a watch-glass add a saturated solution of methyl-violet, drop by drop, until the mixture shows a faint purple-red tinge at the edge of the glass.
1. Stain sections 15-30 minutes.
2. Differentiate in acid alcohol until the purple begins to fade.
3. Wash thoroughly in water.
4. Mount in glycerin. (Sections may be blotted and dehydrated in pure liquid paraffin; the latter is then removed by blotting with xylol, and then mount in pure white vaseline.)
Nuclei are blue, amyloid ruby-red.
8. Kresyl-echt-violett (Morse’s Method).
Kresyl-echt-violett (R. extra) 1 grm., 5 per cent carbolic acid 80 cc., alcohol 20 cc. Mix, stirring well; filter. Solution keeps well, and can be diluted as desired without precipitating.
1. Stain 1-5 minutes.
2. Wash thoroughly in distilled water, differentiating, if necessary.
3. Blot with filter paper.
4. Dehydrate in absolute alcohol as quickly as possible.
5. Clear in turpentine. Blot nearly dry before mounting.
6. Mount in balsam.