Not Staining by Gram’s (Gram-negative).
Gonococcus.
Micrococcus melitensis.
Meningococcus (in sections).
Bacillus of bubonic plague.
Bacillus of chancroid.
Bacillus coli communis.
Bacillus dysenteriæ.
Bacillus of epidemic conjunctivitis (Koch-Weeks).
Bacillus of influenza.
Bacillus mallei.
Bacillus pneumoniæ.
Bacillus proteus.
Bacillus of malignant œdema.
Bacillus pyocyaneus.
Bacillus of typhoid fever.
Bacillus of fowl cholera.
Bacillus of rabbit septicæmia.
Bacillus of swine plague.
Spirillum of Asiatic cholera.
Spirochæte pallida.
3. BACTERIA STAINING BY THE TUBERCLE-BACILLUS METHOD. (ZIEHL-NEELSEN.)
1. Tubercle-bacillus.
2. Lepra-bacillus.
3. Smegma-bacillus.
4. Lustgarten’s bacillus.
1. Stain sections by floating thin paraffin sections directly on to warm carbol-fuchsin (fuchsin 1 grm., absolute alcohol 10 cc., cryst. carbolic acid, 5 grms., water 100 cc.) for 1-3 minutes.
2. Transfer on spatula to water, agitating so as to wash off excess of stain.
3. Transfer section to 30 per cent nitric acid and water alternately, until section has a pale lilac tint.
4. Wash in water.
5. Float on warm watery methylene-blue for 1 minute.
6. Wash in water.