7. Float section on slide; dry over flame or in oven; melt over flame, and put section at once into xylol to remove paraffin.
8. Balsam.
Various staining-methods have been recommended for the staining of the most important pathogenic bacteria in tissues. The most useful of these methods are here given:—
a. Cocci.
1. Pyogenic Cocci. Stain by Gram-Weigert’s, contrast with Bismarck brown or lithium-carmine.
2. Pneumococcus. Stains with ordinary water solutions, carbol-fuchsin and Gram-Weigert’s. The staining of the capsule in sections is not very satisfactory.
3. Gonococcus. Gram-negative. Stains in sections with Zieler’s method, Löffler’s methylene-blue, or dilute carbol-fuchsin with differentiation in alcohol.
4. Micrococcus Catarrhalis. Stains like the gonococcus.
5. Diplococcus Intracellularis Meningitidis. Smear preparations often Gram-positive, in sections usually Gram-negative. Use same stains as for gonococcus.
6. Micrococcus Tetragenus. Stain with Gram’s or watery solutions of basic aniline dyes.