b. Bacilli.
1. Anthrax-bacillus. Stain with Gram-Weigert’s and contrast with Bismarck brown or lithium-carmine. Stains also with strong watery gentian-violet solution, with differentiation in strong alcohol.
2. Bacillus of Malignant Oedema. Gram-negative. Stain with watery solution or gentian-violet.
3. Bacillus of Tetanus. Gram-positive. Stains with watery solutions of basic aniline dyes.
4. Bacillus Aërogenes Capsulatus. Gram-positive. Stains with other aniline stains.
5. Bacillus Pyocyaneus. Stains with Gram’s and other aniline dyes.
6. Bacillus of Influenza. Gram-negative. Fix tissue in alcohol. Stain with dilute carbol-fuchsin and differentiate in dilute acetic acid.
7. Koch-Week’s Bacillus. Gram-negative.
8. Bacillus of Bubonic Plague. Gram-negative. Stain by Gaffky’s method (Fix in alcohol or a mixture of glacial acetic acid 10.0, chloroform 30.0, and 96 per cent alcohol 60.0, imbed in paraffin, stain 2-3 hours in weak watery methylene-blue, dehydrate quickly in absolute alcohol, xylol, balsam). It may also be stained by 24 hours in concentrated solution of fuchsin in glycerin, rapid differentiation in weak acetic; alcohol; xylol; balsam. Alcohol or mercuric chloride fixation should be used, as formol fixation does not give good staining.
9. Typhoid Bacillus. Löffler’s methylene-blue or carbol-fuchsin, staining 24 hours, decolorizing in dilute acetic and washing rapidly in alcohol. Zieler’s method may also be used. It is Gram-negative.