14. Bacillus of Glanders. Gram-negative. Stain with Zieler’s method or with Löffler’s methylene-blue, differentiating in weak acetic.
Noniewicz’s Method.
1. Stain with Löffler’s methylene-blue 2-5 minutes.
2. Wash in water.
3. Differentiate for about 5 seconds in a mixture of ½ per cent acetic acid 75 cc., ½ per cent watery solution tropaeolin 25 cc.
4. Wash in water; dry by blotting; xylol; balsam.
Bacilli deep-blue; tissues light-blue.
15. Bacillus of Rhinoscleroma. Gram-positive. Fix in alcohol for Wolkowitsch’s method:—
1. Stain in aniline gentian-violet 24-48 hours.
2. Wash in water.