3. Treat with Lugol’s 1-4 minutes.
4. Decolorize in absolute alcohol.
5. Remove more color by oil of cloves.
6. Xylol; balsam.
In tissues fixed in osmic acid and then stained in hæmatoxylin the bacilli are dark blue with light blue capsules. The hyaline substance of rhinoscleroma stains with basic stains.
16. Friedländer’s Bacillus. Gram-negative. Stains with ordinary aniline dyes. For staining the capsules the following method is advised:—
1. Stain for 24 hours in the incubator in a mixture of a concentrated alcoholic solution of gentian-violet 50 cc., glacial acetic acid 10 cc., and distilled water 100 cc.
2. Wash in a 1 per cent acetic acid solution.
3. Alcohol; xylol; balsam.
Bacilli deep-blue; capsules light-blue.