3. Wash rapidly in water.
4. Differentiate carefully in absolute alcohol, or in aniline-alcohol (1-10).
5. Carbol xylol; xylol (quickly); balsam.
Nissl’s granules are blue. This method is easy, and the best for general work.
B. Nissl’s Method.
1. Fix in 96 per cent alcohol for 2-5 days; mount tissue in gum arabic on block; harden in 96 per cent alcohol; cut; place sections in 96 per cent alcohol.
2. Stain in methylene-blue soap mixture (methylene-blue B 3.75 grms., Venetian soap shavings 1.75 grms., water 1,000 cc. Shake well. Keep 3 months before using. Shake and filter before using.), warming, until bubbles arise.
3. Differentiate in aniline alcohol (aniline oil 10 parts, 96 per cent alcohol 90 parts) very rapidly.
4. Arrange section on slide; dry with blotting-paper; cover with cajuput oil.
5. Blot; wash off oil with benzene.