6. Remove benzene; mount the wet section in xylol colophonium, slightly warming; press on cover, and remove excess of colophonium.
Nuclei of ganglion cells light blue: granules dark blue. Toluidin blue, thionin, dahlia, Bismarck brown or neutral red may be used instead of methylene-blue, and often give better results.
2. METHODS FOR STAINING MYELIN SHEATHS.
A. Weigert’s Method.
1. Fix in formol 2-3 days.
2. Primary mordant (potassium bichromate 5 grms., fluorchrom 2.5 grms., water 100 cc.: boil and filter) 4-6 days.
3. Without washing after-harden in graded alcohols, in the dark.
4. Imbed in celloidin.
5. Secondary mordant (neutral copper acetate 5 grms., fluorchrom 2.5 grms., water 100 cc., boil and add 36 per cent acetic 5 cc.) for 1 day at 37°C.
6. Transfer to 70-80 per cent alcohol.