3. Imbed in celloidin or paraffin; cut.
4. Remove paraffin; alcohol; water; differentiate in ⅛-1/12 per cent potassium permanganate.
5. Transfer to a 1 per cent oxalic acid solution, until sections become yellowish-green.
6. Wash; dehydrate; xylol; balsam.
Nerves and fat black: tissue yellowish-green. A very reliable method.
3. STAINING OF AXIS CYLINDERS.
Stain with Van Gieson’s (red), Mallory’s aniline blue method (red), lithium carmine (red), or
Williamson’s Modification of Bielschowsky’s Method.
1. Fix in Müller’s; imbed; cut.
2. Place sections in 10 cc. of tap water containing a few drops of formalin, 5 minutes.