3. Wash in water.
4. Place in the following silver bath 5-10 minutes: 3 drops of liq. ammoniæ (B.P.) are dropped into a test tube. Add 10 per cent silver nitrate solution, drop by drop, until a brownish precipitate is formed. Dissolve the latter by adding ammonia, drop by drop, until the fluid is quite clear. Add tap water up to 10 cc.
5. Wash thoroughly in water.
6. Transfer to the dilute formol solution until sections become grayish-black (1-3 minutes).
7. Place in the following solution for a few minutes: To 10 cc. of water add 2 drops of 1 per cent watery solution of chloride of gold, a few drops of saturated borax solution, and a few drops of a 10 per cent solution of potassium carbonate.
8. Transfer to a 10 per cent aqueous solution of sodium hyposulphite for a few minutes.
9. Wash in water; dehydrate in alcohol; clear in oil of cajuput; xylol; balsam.
Axis-cylinders, intracellular fibrils and Golgi’s network are stained.
4. STAINING OF NEURO-FIBRILLAR STRUCTURES.
These are stained by Bielschowsky’s method, and by acid fuchsin after fixation with osmic acid. The special methods (Apathy’s gold method, Bethe’s molybdic method, the silver methods of Ramen y Cajal and Robertson) have little practical application in pathologic work, and are used chiefly in the study of normal histology.