4. Wash rapidly in distilled water.
5. Differentiate in Pal’s solution (oxalic acid 0.5 grm., potassium sulphite 0.5 grm., water 100 cc.) until the normal fibres are entirely decolorized.
6. Dehydrate; xylol; neutral balsam.
Degenerated fibres blue; normal, decolorized.
Method II—
1. Fix in Müller’s fluid: imbed as in Method I.
2. Place sections in 0.5-1 per cent aqueous hæmatoxylin solution, 10-20 minutes.
3. Transfer directly to a saturated aqueous solution of neutral acetate of copper, 30 minutes. Renew copper solution once.
4. Decolorize as in Method I.
5. Wash rapidly in distilled water.