6. Dehydrate in graded alcohols; xylol; balsam.
Degenerated fibres black; normal fibres unstained, except for a narrow circle at periphery.
Method III—
1. Fix and imbed as in Method I.
2. Stain in 0.5-1 per cent aqueous hæmaloxylin solution, 10-20 minutes.
3. Transfer directly to 10-20 per cent solution of perchloride of iron. The section becomes black. After a few seconds they lose their color. If washed in water, they regain their color.
4. Without washing, differentiate in acid alcohol (0.75 cc. HCl in 100 cc. alcohol).
5. Dehydrate in absolute alcohol; xylol; balsam.
Degenerated fibres appear as small black streaks or circular areas.
C. Staining of Fat-granule Cells.