5. Imbed; cut; dehydrate; clear; mount.
Axis cylinders, emerald green. A good method for the rapid demonstration of pathological processes connected with the peripheral nerves.
9. CHROMAFFINIC TISSUES.
Wiesel’s Method.
1. Fix 1-4 days in 10 parts of a 5 per cent potassium bichromate solution, 20 parts of 10 per cent formol, 20 parts distilled water.
2. 1-2 days in 5 per cent potassium bichromate.
3. Wash thoroughly in running water, 24 hours; harden in graded alcohols; imbed in paraffin.
4. Stain sections in a 1 per cent aqueous toluidin-blue or water-blue solution.
5. 5 minutes in tap-water.
6. Stain 20 minutes in a 1 per cent watery safranin solution.