7. 96 per cent, and then absolute alcohol, until the blue color appears.
8. Xylol; balsam.
Chromaffinic cells green; other cells light blue, nuclei red.
X. SKIN.
Skin should be fixed in formol-Müller’s or formol, and should not be left too long in alcohol or xylol. Imbed in celloidin preferably. For general use the ordinary stains suffice; for the study of pigment stain with alum or lithium-carmine. Use Weigert’s elastic-tissue stain for the demonstration of elastic fibrils. The intercellular bridges may be demonstrated by Van Gieson’s (remaining unstained), or by various special staining methods.
Herxheimer’s Method for Epithelial Fibrillae.
1. Harden; imbed; cut.
2. Stain in a saturated watery solution of kresyl-echt-violett.
3. Dehydrate in absolute alcohol; clear in clove oil; balsam.