Leaves, How to Dissect. “For the dissection of leaves,” says Mrs Cussons, “I find the process of maceration too long and tedious, to say nothing of the uncertainty as to the results. I have therefore adopted the use of alkali in saturated solution, the specimens to be introduced while the liquid is heated to the boiling point; the time of immersion to be regulated by the character of the various leaves and the nature of the epidermis to be removed. When the specimen is

freed from epidermis and cellular tissue, it must be subjected to the action of chlorine to destroy the colouring matter. The introduction of peroxide of hydrogen not only serves to render the lace-like specimen purer in colour, but also preserves it. In destroying the colouring matter in ferns this also is invaluable; added to the chlorine it gives a solidity to the bleached fronds, and appears to equalise the action of the chlorine. For skeletonising capsules the slow process of maceration by steeping in rain-water is alone available; a moderate heat may be applied to hasten the process, but alkali is useless. The only known flower which can be dissected is the Hydrangea japonica. The fibrous nature of the petals renders it easy to skeletonise in the perfect truss in which it grows. Skeletonised leaves and capsules appear to gain in the process a toughness and durability not possessed by them in their natural state.”

LECANOR′IC ACID. See Orsellinic acid.

LEECH. Syn. Hirudo (B. P., Ph. L. & D.), L. The officinal leech of the Pharmacopœias is the Sanguisuga medicinalis (Hirudo medicinalis—Cuv.), familiarly known as the ‘old English’ or ‘speckled leech.’ It is also occasionally called the ‘Hamburg grey’ or ‘Russian leech,’ from being imported from those parts. Its characteristics are—Back, greenish or olive green, sometimes almost black or intense brown, with 6 rusty-red or yellowish longitudinal stripes, which are mostly spotted with black.—Belly, dirty yellow or light olive green, spotted more or less with black. The spots are very variable in size and number; in some cases few, in others so numerous as to form the prevailing tint of the belly. This variety, which is the most valuable of the commercial leeches, is chiefly imported from Hamburg.

Another variety of leech, the Sanguisuga officinalis, familiarly known as the ‘Hamburg’ or ‘French green leech,’ is imported from Bordeaux, Lisbon, and Hamburg. Its characteristics are—Back, brownish olive-green, with 6 reddish or rusty yellow longitudinal bands.—Belly, light dirty pea-green, or yellowish green, free from spots, but exhibiting two lateral stripes. This leech is vastly inferior to the preceding variety, and some of those imported from France and Portugal are absolutely useless, from their indisposition to bite, arising from the fraud practised by the collectors and dealers of gorging them with blood to improve their appearance before sending them to market. The above are the species of leech commonly employed in medicine in this country, but many others are noticed by writers on the subject.

Leeches are best preserved in water obtained from a pond, and occasionally changed; when kept in spring water they soon die. The introduction of a hand to which an ill-flavoured medicine or odour adheres into the water in which they are kept is often

sufficient to poison them. The application of saline matter to the skin of leeches, even in very small quantities, immediately occasions the expulsion of the contents of the stomach; hence a few grains of common salt are frequently sprinkled over them, to make them disgorge the blood which they have swallowed. The frequent changing of the water in which leeches are kept is injudicious. Once a month in winter, and once a week in summer, is deemed sufficiently often by the large dealers, unless the water becomes discoloured or bloody, when it should be changed every day, or every other day. When clean pond water cannot be obtained, clean rain water that has been well exposed to the air should alone be employed. Mr J. R. Kenworthy recommends placing in the water a few balls of irregular lumps of pure clay, about 212 inches in diameter; a method which we can recommend as both simple and successful. The plan adopted by M. Fée is as follows:—Place 7 inches of a mixture of moss, turf, and charcoal, in a marble or stone trough, over which sprinkle some small pebbles. At one end of the trough, and about half way up, place a thin shelf of stone or marble, pierced with small holes, on which put first some moss, or portions of marsh horse-tail (Equisetum palustre), and on this a layer of pebbles to keep it down; then pour in water sufficiently high just to moisten the moss and pebbles, put in the leeches, and tie over the mouth of the trough with a cloth. Another plan consists in keeping the leeches in a glass tank, or aquarium, provided with a pebbly bottom and a few healthy aquatic plants.

Propag. According to Dr Wagner, an annual feast on living blood is necessary to render leeches able to grow and propagate. These conditions can only be fulfilled by restoring to the breeding cisterns those which have been already employed. All artificial methods of feeding them by bladders or sponges of blood have been found to fail. He recommends the employment of two tanks, with the bottom formed of loam, clay, or turf, surrounded by an inner border of a similar substance, and an outer one of sand—the one for leeches fit for medical use—and the other for breeding, or for such leeches as have been applied. No leeches are to be taken from the breeding tank until a year has elapsed after their having been applied and fed with human blood; and their removal to the first tank should take place in September, or October, as by this time the breeding season is over. By this plan all leeches that have been applied are to be carefully restored to the breeding tank, without making them disgorge the blood they have swallowed.

LEECH′ING. This consists in the application of leeches to any vascular part of the body, for the purpose of withdrawing blood from it, and thus allaying local inflammation, distension of vessels, &c. Leeches are most

conveniently applied by means of a common pill-box or a wine glass. The part should be previously washed perfectly clean, and if covered with hair should be closely shaved. Sometimes leeches are indisposed to bite; in such cases, allowing them to crawl over a piece of dry linen or calico, rolling them in porter, moistening the part with a little milk or sweetened milk, or drawing a little blood by a slight puncture or scratch, will usually make them bite freely. To stop the bleeding from leech-bites various plans are adopted, among which the application of nitrate of silver or creasote, or gentle pressure for some hours with the finger, are the most successful. Of late years a piece of matico leaf or soldier’s herb, applied in the same manner as a piece of lint, has been commonly adopted to stop the bleeding of leech-bites.