| Agar Hanging-block | Orientation (grouping)............... |
| Chains (No. of elements)............... | |
| Orientation of chains, parallel, irregular. |
| 1. Fermentation-tubes containing peptone-water or sugar-tree bouillon and | Dextrose | Saccharose | Lactose | Maltose | Glycerin | Mannit |
| Gas production, in per cent. | ||||||
| (H/CO2) | ||||||
| Growth in closed arm | ||||||
| Amount of acid produced 1d. | ||||||
| Amount of acid produced 2d. | ||||||
| Amount of acid produced 3d. |
| Substance | Method used | Minutes | Temperature | Killing quantity | Amt. required to restrain growth |
| BRIEF CHARACTERIZATION. Mark + or 0, and when two terms occur on a line erase the one which does not apply unless both apply. | |||
|---|---|---|---|
| MORPHOLOGY[(2)] | Diameter over 1µ | ||
| Chains, filaments | |||
| Endospores | |||
| Capsules | |||
| Zooglea, Pseudozooglea | |||
| Motile | |||
| Involution forms | |||
| Gram’s stain | |||
| CULTURAL FEATURES[(3)] | Broth | Cloudy, turbid | |
| Ring | |||
| Pellicle | |||
| Sediment | |||
| Agar | Shining | ||
| Dull | |||
| Wrinkled | |||
| Chromogenic | |||
| Gel. Plate | Round | ||
| Proteus-like | |||
| Rhizoid | |||
| Filamentous | |||
| Curled | |||
| Gel. Stab. | Surface growth | ||
| Needle growth | |||
| Potato | Moderate, absent | ||
| Abundant | |||
| Discolored | |||
| Starch destroyed | |||
| Grows at 37° C. | |||
| Grows in Cohn’s sol. | |||
| Grows in Uschinsky’s sol. | |||
| BIOCHEMICAL FEATURES | Liquifaction | Gelatin[(4)] | |
| Blood-serum | |||
| Casein | |||
| Milk | Acid curd | ||
| Rennet curd | |||
| Casein peptonized | |||
| Indol[(3)] | |||
| Hydrogen sulphide | |||
| Ammonia[(3)] | |||
| Nitrates reduced[(3)] | |||
| Fluorescent | |||
| Luminous | |||
| DISTRIBUTION | Animal pathogen, epizoon | ||
| Plant pathogen, epiphyte | |||
| Soil | |||
| Milk | |||
| Fresh water | |||
| Salt water | |||
| Sewage | |||
| Iron bacterium | |||
| Sulphur bacterium | |||
FOOTNOTES.
[1] Sir H. A. Blake has called attention to the fact that the “mosquito theory” of malaria is mentioned in a Sanscrit manuscript of about the 6th century A.D.[↩]
[2] Myxomycetes excepted, and they are probably to be regarded as animals—Mycetozoa. [↩]
[3] Centralblatt f. Bakteriologie, etc. LXIII. 1 Abt. Orig. 1912, 4, idem LXVI. 1 Abt. Orig. 1912, 323. [↩]
[4] The pronunciation of this word according to English standards is kok-si; the continental pronunciation is kok-kee; the commonest American seems to be kok-ki. We prefer the latter since it is easier and more natural and should like to see it adopted. (Author.) [↩]
[5] With the possible exception of blue green algæ which have been found with bacteria in the above-mentioned hot springs. Seeds of many plants have been subjected to as low temperatures as those above-mentioned without apparent injury. [↩]