Collecting is an important means of ecological study. This is particularly true in the early stages of such study, but as the student becomes familiar with species and comes to know many of them at sight, less collecting will be necessary, except in the case of very small species and in those studies which depend upon the total catch as a means of securing data, as, for example, the case of plankton organisms. On account of the large number of species, very few students will be able to determine them at sight, but this does not disqualify a student for beginning ecological study. The greatest aid in handling such a varied population is a numbering of the individuals, or lots of specimens, consecutively and recording the full data for them in the notebook. The question arises at once as to how many specimens are to be assigned to one number. No rigid rule can be followed, but in general it is safe to assign a single number to all specimens which agree in all the fundamental data, as date, place of capture, and exact habitat. And for my own part I number all individuals taken, upon which any special observations are made, as when a Phymatid is taken with a dying or dead honeybee, or a dragon fly is taken from the web of an Argiope. In this way the confusion which is particularly liable to creep into one’s work, where he deals with a large number of species, and before familiarity with them is acquired, may be reduced to the minimum. For small animals I have found useful a very liberal use of vials, into which field numbers, corresponding to numbers in the field notes, are placed immediately upon capture of the specimens, and not minutes or hours later when the vials have become mixed, and recollection is less sharp. Specimens which have been thoroughly numbered may thus be sent to specialists for determination. This method uses up many numbers, but, fortunately, the supply is unlimited, and it gives greater precision to one’s notes, and encourages detailed observations on individual animals.

In the eyes of many the determination of specimens is such a formidable task that they are at once repelled from any subject which involves numerous species. But as we have previously remarked, there are phases of ecological study which involve only a minimum amount of such work. On the other hand, one may readily have an undue fear of numerous species, but no one can doubt that the smaller number of species found upon mountains or in the far north gives to a beginner certain advantages for study. But if one’s studies are confined to the more restricted habitats, the number of species involved is comparable to the more favored localities mentioned.

An accurately determined series of specimens, conveniently arranged, will be an important aid in one’s studies. Two forms of arrangement of such determined series are very helpful; one being synoptic or systematically arranged, and the other ecologically, by associations or by a topical arrangement in harmony with the subjects being investigated. With the use of such series and proper caution, aided by the best keys in the literature, one may hope to make many of his own determinations and thus economize his time.

There are many ways to secure the initial series of determined specimens, and some of these are the following: Our governmental departments, both national and state, and many of our large museums, universities, and academies, have numerous experts who are quite willing and even eager to aid earnest students who wish to have specimens determined. Then, in addition, there are many expert amateurs who are equally liberal, so that when all the sources of aid are considered, the list becomes a surprisingly long one. This is the fact that should be called to mind when considering large numbers of species. A student therefore does not need to work alone, but may have the coöperation of a large number of able and willing collaborators. Naturally we turn for aid to our United States National Museum as one of the first sources of assistance, to the Smithsonian Institution, and to the various scientific departments of the U. S. Department of Agriculture, particularly to the Bureaus of Plant Industry, Biological Survey, and Entomology. These sources alone are able to determine almost any reasonable series of specimens, particularly if they are well preserved. Arrangements for such determinations can probably be made by addressing the proper authorities.

The museums of our larger cities, as the American Museum of Natural History of New York, the Carnegie Museum at Pittsburgh, the Field Museum of Chicago, and many smaller state and local museums are able to give very efficient aid in this line. Other local institutions are the State Universities and Experiment Stations, and the local natural history surveys, which often exist under the guise of a geological or agricultural organization. Frequently they are qualified and willing to do this work.

In very difficult cases it may be necessary to have recourse to Cassell’s Naturalist’s Universal Directory (Boston, 1905), in order to find the address of some specialist in a foreign land, who can help, but generally Americans are the best informed upon their own fauna.

Supplementary to, and in some cases a necessary substitute for, a reference series of authentically determined specimens, is one which the student is able to name for himself, by the study of the literature. Without some special training this may become dangerous ground to tread upon, but every now and then some young student begins in this way and develops such care in determining his own collections, that the method cannot be wholly condemned. Such work in itself has fascinations, and one may easily occupy all one’s time with it. From the ecological standpoint to stop with determinations only would be like acquiring a certain vocabulary, and stopping before learning how to use the language. Ecological work aims to use the names of species as the most concise method of referring to kinds of animals whose interrelations are to be described and interpreted. Taxonomy is the tool.

V. REFERENCES TO SCIENTIFIC TECHNIQUE

1. The Scientific Method.

2. Directions for Collecting and Preserving Specimens, Photographing, Surveying, and Other Phases of Technique.