This small amount of milk may be pasteurized by placing the container in water heated to the desired temperature. A very satisfactory arrangement is to cut of a barrel, and place a steam pipe in it. The barrel can then be filled partly full of water and heated by steam. The bottles of milk to be pasteurized are hung in the water in the barrel. Two or three more bottles should be prepared than it is expected will be used as some of the bottles are liable to be broken while cooling or heating. The bottles should be filled about two-thirds full. This leaves room enough to add the mother starter and later to break up the starter to examine it. It is desirable not to have the milk or starter touch the cover since contaminations are more likely. It is a good plan when pasteurizing to have one bottle as a check. This may be filled with water and left open and the thermometer placed in it. A uniform temperature may be obtained by shaking the bottles.
64. Adding cultures.—After being pasteurized, the milk should be cooled to a temperature of 80° F. This is a suitable temperature for the development of the lactic acid-forming organisms. The commercial or pure culture should now be added to the milk at the rate specified in the directions. Care should be exercised in opening bottles not to put the covers in an unclean place. A sterile dipper is a good place to put them. After the culture has been added to the milk, it should be mixed thoroughly by shaking the bottle. This should be repeated every fifteen or twenty minutes for four or five times. This is done to make certain that the culture is thoroughly mixed with the milk. The milk should be placed in a room or incubator as near 80° F. as possible, in order to have a uniform temperature for the growth of the organisms. The bacteria in the pure culture are more or less dormant so that a somewhat higher temperature than the ordinary is necessary to stimulate their activity. This milk should be coagulated in eighteen to twenty-four hours, depending largely on the uniformity of the temperature during incubation.
65. Cleanliness.—To produce a good starter, great care should be exercised that all utensils coming in contact with the milk are sterile. After the milk is in the container in which the starter is made, it should be kept covered as continuously as possible. Thermometers should not be put into it to ascertain the temperature. When examining the starter, do not dip into it, but pour out, as this prevents contamination. The cover, when removed from the container, should be put in a sterile place in such way that the dirt will not stick to it and later get into the starter.
66. "Mother" starter or startoline.—The thickened sour milk obtained by inoculating the sweet pasteurized milk with pure culture of lactic acid-forming bacteria is known as "mother starter" or "startoline."
67. Examining starter.—This starter should be examined carefully as to physical properties, odor and taste. The coagulation should be smooth, free from whey and gassy pockets or bubbles. Sometimes the first few inoculations from a new culture will show signs of gas, but, usually, this will quickly disappear, and not injure the starter. It should have a clean sour cream odor and clean, mild, acid flavor. After breaking up it should be thick and creamy, entirely free from lumps. This starter may have an objectionable flavor, due to the media in which the organisms were growing when shipped. In such cases it is necessary to carry the starter one or two propagations to overcome the flavor, to enliven the micro-organisms and to secure the quantity desired.
68. Second day's propagation.—For the second day, the milk for the starter is selected as on the first day. It is pasteurized, and this time cooled to 70° F. The milk is cooled a trifle colder the second day than the first, because the organisms have become more active and hence do not require as high a temperature to grow. Instead of inoculating with powder, as was done the first day, the mother starter prepared the first day is used. This requires only a very small amount, perhaps a tablespoonful to a quart bottle. It should be thoroughly mixed with the milk. This starter may have the
Fig. 6.—An improved starter-can. flavor of the media used in the laboratory culture, therefore may need to be carried one or two days more to eliminate it. After the flavor has become normal, the mother starter is ready for commercial use.
69. Preparation of larger amount of starter.—The first thing to determine is the quantity of starter required. As much milk should be carefully chosen as the amount of starter desired. This milk should then be pasteurized. An improved starter-can ([Fig. 6]) may be used in the pasteurization of the milk and the making of starter, or a milk-can ([Fig. 7]) placed in a tub of water in which there is a steam pipe. The former requires mechanical power to operate the agitator, but the latter can be used where mechanical power is not available. In the latter the milk and starter are stirred by hand. This is the kind of apparatus more
