In making dilutions you may use your syringe instead of pipette.

Dilutions can be kept one week in a dark, cool place.

Discard turbid solutions.

4. Preparation of the Patient for the Test:

Patient to keep quiet in bed, or reclining chair, for two or three days before injection.

Take temperature every two or three hours for two or three days (daytime).

If the test is to be applied, highest temperature should not be above 99.1 F, by mouth, according to Koch; not above 100 F, according to others.

Site of injection—back, below the level of the shoulder blades, alternately on the two sides.

Rub skin with ether or alcohol.

An exact record of physical signs, just before injection, should be made by the physician.