Typhoid in bouillon, 10 per cent. of chlorlyptus.
Staphylococci in pus, 5 per cent. of chlorlyptus.
Staphylococci in serum, 1 per cent. of chlorlyptus.
It seems to the referee that a substance that is ineffective with an hour’s exposure to these concentrations is not at all likely to kill or check bacteria under clinical conditions. In other words, it is not an antiseptic in the ordinary sense.
The referee is not impressed by the superior power attributed by Rivas to chlorlyptus in the presence of pus. Inefficiency of 10 per cent. for one-half hour or of 5 per cent. for two hours seems a failure rather than a success. The referee also notes the absence of any data as to the relative efficiency of chlorlyptus against staphylococci in pus and in bouillon. The data on serum indicate that chlorlyptus is much weaker than phenol and show that it is less effective in the presence of pus than in other mediums.
The referee fails to grasp the bearing of the oil experiments on any clinical condition. Moreover, the inconstant results mentioned by Rivas suggest the possibility that the incorporation of the bacteria in oil may have prevented their effective distribution in the culture medium. If any significance is to be attached to these experiments, they should be checked by controls, without antiseptics.
SUMMARY OF RIVAS’ IN VITRO EXPERIMENTS
| Minimal Germicidal Concentrations | Maximal Not Germicidal Concentrations | |
| Typhoid Bacilli in Bouillon: | ||
| Chlorlyptus (Exp. 3) | 10%, 2 to 4 hours | 10% for 1 hour |
| 5% for 2 hours | ||
| Eucalyptus oil (Exp. 1) | 5% within 5 minutes | No data |
| Phenol (Exp. 5) | 1% within 10 min. | No data |
| Streptococci and Staphylococci in Olive Oil: | ||
| Chlorlyptus (Exps. 7 and 8) | 1%, almost at once, sometimes | No data |
| Eucalyptus oil | No data | No data |
| Phenol (Exps. 9 and 10) | 1%, almost at once, | No data |
| Staphylococci in Pus: | ||
| Chlorlyptus (Exp. 11) | 10% for 1 hour | 10% for 1⁄2 hour |
| 5% for 2 hours | ||
| Eucalyptus oil | No data | No data |
| Phenol | No data | No data |
| Staphylococci in Human Blood Serum: | ||
| Chlorlyptus (Exp. 12) | 5% in 1 hour | 1% in 1 hour |
| Eucalyptus oil | No data | No data |
| Phenol | 5% almost at once | 1% in 1 hour |
INFECTION EXPERIMENTS IN VIVO
Dr. Rivas reports two series of experiments, in each of which three guinea-pigs received staphylococcus suspensions in the peritoneum. One guinea-pig in each series was left untreated; the others received injections of chlorlyptus into the peritoneum at various intervals.
The following results were obtained:
| Chlorlyptus | Results | |
| Exp. 19, No. 1 | None | Survived |
| Exp. 20, No. 1 | None | Died |
| Exp. 19, No. 2 | At once | Died |
| Exp. 19, No. 3 | After 24 hours | Survived |
| Exp. 20, No. 2 | After 18 hours | Died |
| Exp. 20, No. 3 | After 24 hours | Died |