CHLORLYPTUS: REACTION (LITMUS PAPER) ON CONTACT WITH TISSUE
| Serial No. | Animal | When Injected | Quantity, C.c. | Time of Death | Blue Litmus | Symptoms or Toxicity |
| 1 | Rat | Pleura | 1 | 1⁄2 hour | Remains blue | None; killed; pleura not congested; lung spec. = 21; slight congestion |
| 2 | Rat | Pleura | Less than 1 | 1 hour | Remains blue | Negative |
| 3 | Rat | Pleura | 1 | 23 min. | Remains blue | Almost at once bad gasping respiration and died in 23 m.; heart distend.; possibly injection penetrated lung |
| Peritoneum | 1 | 23 min. | Turns red | |||
| 4 | Rabbit | Pleura | 1 | ........ | ........ | Died overnight |
| 5 | Dog | Pleura | 1 | 1⁄4 hour | Remains blue 20 m. p. m. | |
| Peritoneum | 1 | 1⁄4 hour | Remains blue 20 m. p. m. | |||
| 6 | Dog | Pleura | 1 | 3 min. | Remains blue 45 m. p. m. | |
| Peritoneum | 1 | 3 min. | Remains blue 45 m. p. m. | |||
| 7 | Dog | Pleura | 1 | 20 min. | Remains blue 20 m. p. m. | |
| Peritoneum | 1 | 20 min. | Remains blue 20 m. p. m. |
SERIES B: SEROUS MEMBRANES
In these experiments, 1 c.c. of chlorlyptus was injected into the pleura or peritoneum. After a stated time, the animal was killed, and the reaction of the pleural or peritoneal surface was tested with blue litmus paper. The results are shown in the table.
C. TOXICITY EXPERIMENTS
By the Referee
TECHNIC
White rats were injected hypodermically with chlorlyptus or with eucalyptus oil, diluted with olive oil in the ratio of 1:4. The larger doses were divided between two or more sites of injection.