DETAILED PROTOCOLS
Hypodermic injections in white rats. Drugs diluted with 3 parts of olive oil. Doses are given as cubic centimeters of pure drug per kilogram of rat.
A. EUCALYPTUS SERIES
Experiment 1.—1.25 c.c.; injected VII.9.19: Active; walks about. No depression at any time. VII.10.19. Appears normal.
Experiment 2.—2.5 c.c.; injected VI.30.19: Quiet—not very depressed, reflexes good (six hours).
VII.1.19—Active—reflexes good, eats moderately.
VII.2.19—Animal acts normal—eats moderately, reflexes good; active (a.m.). Later in day, depressed.
VII.4.19—Died during night of VII.3.19.
Experiment 3.—3.75 c.c.; injected VI.24.19: Quiet; depressed; pain reflex diminished. Animal lay on ventral surface, not supported by legs. Will get on to feet very sluggishly if turned on side (twenty-four hours). Does not eat.
VI.26.19—Depressed slightly; pain reflex present.
VI.27.19—Fairly active; eats a little.
VI.28.19—Depressed.
Died during night of VI.29.19 (three days).
Experiment 4.—5 c.c.; injected VI.24.19: Quiet; markedly depressed (one hour). Does not get on feet when turned on side; ataxia well marked. Slight watery secretion in eyes. Reflexes diminished. Does not eat (twenty-four hours).
VI.26.19—Heart slowed and arrhythmic. Animal lies on side. Unable to walk; markedly depressed.
VI.27.19—Lies on side; does not eat. Died during night of VI.27.19 (three days).
Experiment 5.—6.25 c.c.; injected VI.24.19: Quiet; very markedly depressed. Heart and respiration greatly slowed. Lies on side; tears in eyes; does not eat (twenty-four hours).
VI.25.19—Temperature subnormal; cold to touch; tail stiffened and straight.
Died during night of VI.25.19 (one and one-half days).
Postmortem: Lungs congested. Liver pale in color. Spleen very dark red. Kidneys normal. Other organs normal.
B. CHLORLYPTUS EXPERIMENTS
Experiment 1.—1.56 c.c.; injected VI.24.19: Rather restless for an hour. Active during next four hours and following twenty-four. Eats well, reflexes good. Acts normal on VII.1.19 and since VI.26.19.