Experiment 2.—3.75 c.c.; injected VI.24.19: More quiet; active during next twenty-four hours. Reflex all right. Eats well; normal VII.1.19, since VI.26.19.
Experiment 3.—5 c.c.; injected VI.24.19: Quiet; defecation in four hours. Rather quiet for six hours. Eats well. Reflexes good; normal VII.1.19, since VI.26.19.
Experiment 4.—6.25 c.c.; injected VI.24.19: Quiet and breathing labored in four hours; active after twenty-four hours. Eats well. Somewhat depressed on VI.26.19; pain reflex present. On VI.26.19, eats well and fairly active. Active and eats, VI.27.19. Appears normal, VII.1.19.
Experiment 5.—8.75 c.c.; injected VI.30.19: Rather quiet during next two hours. Morning of VII.1.19, lies on stomach; quiet; does not eat very much. Pain reflexes good. VII.2.19, still depressed; does not eat. Appears normal, VII.3.19.
Experiment 6.—12.5 c.c.; injected VI.25.19: Quiet, but reflexes good; more quiet and depressed after several hours. Some loss of oil from wound. Died night of VI.25.19 (one day). Tail stiff. Temperature low.
Postmortem: Lungs markedly congested. Spleen and liver dark red. One kidney congested. Other viscera normal.
Experiment 7.—12.5 c.c.; injected VII.9.19: Quiet for one-half hour; 1.5 hours twitching of muscles of whole body, lies on side, ataxia present. Died night of VII.9.19 (one day).
Experiment 8.—18.75 c.c.; injected VI.25.19: Quiet; reflexes good (three hours). Some loss of oil. Depressed and turns on side (six hours). Died night of VI.25.19 (one day).
Postmortem: Lungs congested. Spleen and liver very dark red. Right kidney much darker red. Viscera normal.
D. REPORT OF DR. D. RIVAS
The following are the results of experiments conducted by me, during the past four months, on the germicidal action of chlorlyptus (chlorinated oil of eucalyptus, principal constituent C10H17OCl2) in vitro and in vivo, and comparison also with carbolic acid, oil of eucalyptus and dichloramine in test for irritation and toxicity.