Experiment 7.—Germicidal action of chlorlyptus on pyogenic bacteria suspended in an oily medium.—Experiment with strepto­coccus: Cultures of strepto­coccus in blood agar three days old were suspended in olive oil (sterile), and chlorlyptus was added in the proportions of 1, 5 and 10 per cent. and inoculated in trypsinized bouillon at different intervals, namely: at once, after five minutes, after ten minutes, after fifteen minutes, after thirty minutes, and after one hour. Tubes were incubated at 37 C. for forty-eight hours.
Result: All tubes remained sterile. The germicidal action of chlorlyptus on strepto­coccus suspended in oil was almost at once and with certainty after five minutes when added in the proportion of 1, 5 and 10 per cent.

Experiment 8.—Germicidal action of chlorlyptus on staphylo­coccus, suspended in sterile olive oil.—The technic employed was the same as in Experiment 5, except that a culture of staphylo­coccus was used.
Result: All tubes remained sterile. The germicidal action of chlorlyptus was almost at once in the proportions of 1, 5 and 10 per cent.
Remarks: By repeating this experiment the result showed some variations. The discrepancy was probably due to an imperfect suspension of the micro-organism in the oil.

Experiment 9.—Germicidal action of carbolic acid on strepto­coccus suspended in olive oil.—The technic employed was the same as in Experiment 5, except that carbolic acid was used instead of chlorlyptus.
Result: The germicidal action of carbolic acid of strepto­coccus suspended in olive oil was almost at once in the proportions of 1, 5 and 10.

Experiment 10.—Germicidal action of chlorlyptus on staphylo­coccus.—The technic employed was the same as in Experiment 6 except that the carbolic acid was used instead of chlorlyptus.
Result: The germicidal action of carbolic acid on staphylo­coccus suspended in olive oil was almost at once, in proportions of 1, 5 and 10 per cent.

Experiment 11.—Germicidal action of chlorlyptus on pyogenic bacteria suspended in pus.—Chlorlyptus was added to sterile pus in the proportions of 1, 5 and 10 per cent., and then inoculated with staphylo­coccus and cultures were made in bouillon at once, after five minutes, after ten minutes, after fifteen minutes, after thirty minutes, after one hour and after two hours, respectively, and tubes incubated for forty-eight hours at 37 C.
Result: Growth was shown in all tubes except those inoculated from tubes in which chlorlyptus was added in the proportions of 10 per cent. after one hour.

Experiment 12.—Germicidal action of chlorlyptus on strepto­coccus suspended in sterile human blood serum.—Staphylo­coccus culture in agar forty-eight hours old was suspended in sterile human blood serum, and to the suspension chlorlyptus 5 per cent. in paraffin oil was added in the proportions of 1, 5 and 10 per cent. Inoculations were made at intervals, at once, after five minutes, after ten minutes, after fifteen minutes and after one hour in trypsinized bouillon. Tubes were incubated at 37 C. for forty-eight hours.
Result: Chlorlyptus showed inhibitory action on the growth of staphylo­coccus in the strength of 10 per cent., but did not produce complete sterilization. Similar results were shown with the 5 per cent., and in the 1 per cent. chlorlyptus did not show any inhibitory action at all.

Experiment 13.—Germicidal action of carbolic acid on staphylo­coccus suspended in human blood serum (sterile).—The technic employed was the same as in Experiment 10 except that carbolic acid was used instead of chlorlyptus.
Result: Carbolic acid produced a complete sterilization in the strength of 10 per cent. almost at once, and with certainty after five minutes. Similar results were produced with the 5 per cent. The 1 per cent. carbolic acid did not show any appreciable germicidal action on staphylo­coccus.

Experiment 14.—Toxic and irritant action of chlorlyptus.—Six normal guinea-pigs were used for the experiment. Guinea-Pig 1 was injected peritoneally with 1 c.c. of chlorlyptus, Guinea-Pig 2 with 2 c.c. of chlorlyptus, Guinea-Pig 3 with 3 c.c. of chlorlyptus, Guinea-Pig 4 with 4 c.c. and Guinea-Pig 5 with 5 c.c. 5 per cent. respectively. Guinea-Pig 6 was used as a control and not injected.
 Result: Guinea-Pigs 1 and 2 did not show any appreciable disturbance. Guinea-Pig 3 was sick for four days, after which it gradually recovered but it became sick again after one week and died ten days after the injection. Guinea-Pig 4 died over night. Guinea-Pig 5 died six hours after injection. Guinea-Pig 5 was injected at 11:30 with 5 c.c. chlorlyptus. Ten minutes after the injection it was lying relaxed, respiration and heart normal, conjunctive reflex present. One hour after the injection the animal seemed to present symptoms resembling those of narcosis: respiration and heart were normal. After four hours there was no change in the condition of the guinea-pig except that the respiration was irregular. Five and a half hours after it showed prostration with irregular respiration and heart action. Six hours after injection the animal was dead.
Autopsy: The peritoneum showed a congestion and a fibrinous exudation, amount of liquid increased, some part of which was probably chlorlyptus unabsorbed. Spleen about normal, liver congested, kidney about normal, suprarenal glands about normal, lungs normal, pleural cavity obtained no exudation, heart soft, flabby and congested.

Experiment 15.—Toxic and irritant action of chlorlyptus when injected into the pleural cavity.—Six normal guinea-pigs used for the experiment. Chlorlyptus was injected in the pleural cavity as follows: Guinea-Pig 1, 0.5 c.c.; Guinea-Pig 2, 1 c.c.; Guinea-Pig 3, 2 c.c.; Guinea-Pig 4, 3 c.c., and Guinea-Pig 5, 4 c.c. Guinea-Pig 6 was used as a control.
Result: Guinea-Pigs 1 and 2 recovered about four hours after injection. Guinea-Pig 3 died three days after and Guinea-Pigs 4 and 5 four and two hours after, respectively.
Conclusions: Guinea-pigs weighing on the average of 400 gm. may be injected peritoneally with one or two c.c. or intrapleurally with 0.5 to 1 c.c. of chlorlyptus without having fatal results from the injection.

Experiment 16.—Toxic and irritant action of eucalyptus oil.—Three normal guinea-pigs were used for the experiment. Guinea-Pig 1 was injected with 1 c.c. of oil of eucalyptus in the peritoneum, and Guinea-Pig 2 with 0.5 c.c. in the pleural cavity. Guinea-Pig 3 was used as a control.
Result: Guinea-Pig 1 died about three hours after injection, and Guinea-Pig 2 about two hours after the injection.
Autopsy: Both guinea-pigs showed marked congestion and a moderate degree of exudate in the peritoneum.