1. The protective inoculation consists in the intravenous injection of a single immunizing unit of tubercle germs (derived from man), rubbed up with sterile salt solution; and in a second intravenous injection, three months later, of five immunizing units of the same germs.
2. The germs in question can be obtained in glass tubes containing 5 or 20 units.
3. As a rule only cattle without external manifestations of disease, aged from three weeks to four months (at the first inoculation) should be inoculated. With healthy animals of this age it is unnecessary to test them previously with tuberculin, even if they belong to a notoriously tubercular herd.
4. In exceptional cases older animals (from four months to two years) may be inoculated with the virus, but only when they are entirely free from disease, and when a tuberculin test made on them results without any reaction whatsoever.
5. The inoculated animals are to be kept in their stalls two days before and two weeks after the inoculation.
6. In all cases in which the owners can manage the rectal temperatures, the same are to be taken two days before the inoculation (morning and evening) and again on the morning of the inoculation. After this they are taken once more in the evening and then once daily for the next five days. If, after this, the animals still have temperatures above 39.2° C., the temperatures are to be taken daily until the same reach 39.2° C.
7. In animals not over four months old at the time of the first inoculation, the temperature may be omitted if there be any difficulties in taking it. In those, however, which are over that age at the time of the first inoculation, the temperature must invariably be recorded according to paragraph 6. Even when inoculating animals in infected herds the above-mentioned measurements should be taken whenever practicable.
8. If possible, the inoculated animals should be weighed every fourteen days on a cattle scale.
9. Owners of cattle are to keep record of all the temperatures and weights. You are to collect the records concerning temperatures as soon as they have been completed; the records of the weights, however, only after three months. The results noted in the temperature returns are to be entered by you on the charts herewith provided, and a temperature curve plotted. These charts are to be made in duplicate, one copy to be sent to our representative and the other to the Experimental Division of the Hygienic Institute, Marburg.
10. The inoculated animals are all to be permanently marked. Those on which temperature and weight observations have been made should also be numbered.