140. Allihn’s Gravimetric Method for the Determination of Dextrose.—Reagents:
| I. | 34.639 grams of CuSO₄.5H₂O, | dissolved in water and diluted
to half a liter: |
| II. | 173 grams of rochelle salts | dissolved in water and diluted |
| | 125 grams of KOH, | to half a liter. |
Manipulation: Place thirty cubic centimeters of the copper solution (I), thirty cubic centimeters of the alkaline tartrate solution (II), and sixty cubic centimeters of water in a beaker and heat to boiling. Add twenty-five cubic centimeters of the solution of the material to be examined, which must be so prepared as not to contain more than one per cent of dextrose, and boil for two minutes. Filter immediately after adding an equal volume of recently boiled cold water and obtain the weight of copper by one of the gravimetric methods given. The corresponding weight of dextrose is found by the following table:
Allihn’s Table for the
Determination of Dextrose.
- (A) = Milligrams of copper.
- (B) = Milligrams of dextrose.
| (A) | (B) | (A) | (B) | (A) | (B) | (A) | (B) | (A) | (B) |
|---|
| 10 | 6.1 | 46 | 23.9 | 82 | 41.8 | 118 | 60.1 | 154 | 78.6 |
| 11 | 6.6 | 47 | 24.4 | 83 | 42.3 | 119 | 60.6 | 155 | 79.1 |
| 12 | 7.1 | 48 | 24.9 | 84 | 42.8 | 120 | 61.1 | 156 | 79.6 |
| 13 | 7.6 | 49 | 25.4 | 85 | 43.4 | 121 | 61.6 | 157 | 80.1 |
| 14 | 8.1 | 50 | 25.9 | 86 | 43.9 | 122 | 62.1 | 158 | 80.7 |
| 15 | 8.6 | 51 | 26.4 | 87 | 44.4 | 123 | 62.6 | 159 | 81.2 |
| 16 | 9.0 | 52 | 26.9 | 88 | 44.9 | 124 | 63.1 | 160 | 81.7 |
| 17 | 9.5 | 53 | 27.4 | 89 | 45.4 | 125 | 63.7 | 161 | 82.2 |
| 18 | 10.0 | 54 | 27.9 | 90 | 45.9 | 126 | 64.2 | 162 | 82.7 |
| 19 | 10.5 | 55 | 28.4 | 91 | 46.4 | 127 | 64.7 | 163 | 83.3 |
| 20 | 11.0 | 56 | 28.8 | 92 | 46.9 | 128 | 65.2 | 164 | 83.8 |
| 21 | 11.5 | 57 | 29.3 | 93 | 47.4 | 129 | 65.7 | 165 | 84.3 |
| 22 | 12.0 | 58 | 29.8 | 94 | 47.9 | 130 | 66.2 | 166 | 84.8 |
| 23 | 12.5 | 59 | 30.3 | 95 | 48.4 | 131 | 66.7 | 167 | 85.3 |
| 24 | 13.0 | 60 | 30.8 | 96 | 48.9 | 132 | 67.2 | 168 | 85.9 |
| 25 | 13.5 | 61 | 31.3 | 97 | 49.4 | 133 | 67.7 | 169 | 86.4 |
| 26 | 14.0 | 62 | 31.8 | 98 | 49.9 | 134 | 68.2 | 170 | 86.9 |
| 27 | 14.5 | 63 | 32.3 | 99 | 50.4 | 135 | 68.8 | 171 | 87.4 |
| 28 | 15.0 | 64 | 32.8 | 100 | 50.9 | 136 | 69.3 | 172 | 87.9 |
| 29 | 15.5 | 65 | 33.3 | 101 | 51.4 | 137 | 69.8 | 173 | 88.5 |
| 30 | 16.0 | 66 | 33.8 | 102 | 51.9 | 138 | 70.3 | 174 | 89.0 |
| 31 | 16.5 | 67 | 34.3 | 103 | 52.4 | 139 | 70.8 | 175 | 89.5 |
| 32 | 17.0 | 68 | 34.8 | 104 | 52.9 | 140 | 71.3 | 176 | 90.0 |
| 33 | 17.5 | 69 | 35.3 | 105 | 53.5 | 141 | 71.8 | 177 | 90.5 |
| 34 | 18.0 | 70 | 35.8 | 106 | 54.0 | 142 | 72.3 | 178 | 91.1 |
| 35 | 18.5 | 71 | 36.3 | 107 | 54.5 | 143 | 72.9 | 179 | 91.6 |
| 36 | 18.9 | 72 | 36.8 | 108 | 55.0 | 144 | 73.4 | 180 | 92.1 |
| 37 | 19.4 | 73 | 37.3 | 109 | 55.5 | 145 | 73.9 | 181 | 92.6 |
| 38 | 19.9 | 74 | 37.8 | 110 | 56.0 | 146 | 74.4 | 182 | 93.1 |
| 39 | 20.4 | 75 | 38.3 | 111 | 56.5 | 147 | 74.9 | 183 | 93.7 |
| 40 | 20.9 | 76 | 38.8 | 112 | 57.0 | 148 | 75.5 | 184 | 94.2 |
| 41 | 21.4 | 77 | 39.3 | 113 | 57.5 | 149 | 76.0 | 185 | 94.7 |
| 42 | 21.9 | 78 | 39.8 | 114 | 58.0 | 150 | 76.5 | 186 | 95.2 |
| 43 | 22.4 | 79 | 40.3 | 115 | 58.6 | 151 | 77.0 | 187 | 95.7 |
| 44 | 22.9 | 80 | 40.8 | 116 | 59.1 | 152 | 77.5 | 188 | 96.3 |
| 45 | 23.4 | 81 | 41.3 | 117 | 59.6 | 153 | 78.1 | 189 | 96.8 |
| |
| (A) | (B) | (A) | (B) | (A) | (B) | (A) | (B) | (A) | (B) |
| 190 | 97.3 | 233 | 120.1 | 276 | 143.3 | 319 | 167.0 | 362 | 191.1 |
| 191 | 97.8 | 234 | 120.7 | 277 | 143.9 | 320 | 167.5 | 363 | 191.7 |
| 192 | 98.4 | 235 | 121.2 | 278 | 144.4 | 321 | 168.1 | 364 | 192.3 |
| 193 | 98.9 | 236 | 121.7 | 279 | 145.0 | 322 | 168.6 | 365 | 192.9 |
| 194 | 99.4 | 237 | 122.3 | 280 | 145.5 | 323 | 169.2 | 366 | 193.4 |
| 195 | 100.0 | 238 | 122.8 | 281 | 146.1 | 324 | 169.7 | 367 | 194.0 |
| 196 | 100.5 | 239 | 123.4 | 282 | 146.6 | 325 | 170.3 | 368 | 194.6 |
| 197 | 101.0 | 240 | 123.9 | 283 | 147.2 | 326 | 170.9 | 369 | 195.1 |
| 198 | 101.5 | 241 | 124.4 | 284 | 147.7 | 327 | 171.4 | 370 | 195.7 |
| 199 | 102.0 | 242 | 125.0 | 285 | 148.3 | 328 | 172.0 | 371 | 196.3 |
| 200 | 102.6 | 243 | 125.5 | 286 | 148.8 | 329 | 172.5 | 372 | 196.8 |
| 201 | 103.1 | 244 | 126.0 | 287 | 149.5 | 330 | 173.1 | 373 | 197.4 |
| 202 | 103.7 | 245 | 126.6 | 288 | 149.4 | 331 | 173.7 | 374 | 198.0 |
| 203 | 104.2 | 246 | 127.1 | 289 | 150.9 | 332 | 174.2 | 375 | 198.6 |
| 204 | 104.7 | 247 | 127.6 | 290 | 151.0 | 333 | 174.8 | 376 | 199.1 |
| 205 | 105.3 | 248 | 128.1 | 291 | 151.6 | 334 | 175.3 | 377 | 199.7 |
| 206 | 105.8 | 249 | 128.7 | 292 | 152.1 | 335 | 175.9 | 378 | 200.3 |
| 207 | 106.3 | 250 | 129.2 | 293 | 152.7 | 336 | 176.5 | 379 | 200.8 |
| 208 | 106.8 | 251 | 129.7 | 294 | 153.2 | 337 | 177.0 | 380 | 201.4 |
| 209 | 107.4 | 252 | 130.3 | 295 | 153.8 | 338 | 177.6 | 381 | 202.0 |
| 210 | 107.9 | 253 | 130.8 | 296 | 154.3 | 339 | 178.1 | 382 | 202.5 |
| 211 | 108.4 | 254 | 131.4 | 297 | 154.9 | 340 | 178.7 | 383 | 203.1 |
| 212 | 109.0 | 255 | 131.9 | 298 | 155.4 | 341 | 179.3 | 384 | 203.7 |
| 213 | 109.5 | 256 | 132.4 | 299 | 156.0 | 342 | 179.8 | 385 | 204.3 |
| 214 | 110.0 | 257 | 133.0 | 300 | 156.5 | 343 | 180.4 | 386 | 204.8 |
| 215 | 110.6 | 258 | 133.5 | 301 | 157.1 | 344 | 180.9 | 387 | 205.4 |
| 216 | 111.1 | 259 | 134.1 | 302 | 157.6 | 345 | 181.5 | 388 | 206.0 |
| 217 | 111.6 | 260 | 134.6 | 303 | 158.2 | 346 | 182.1 | 389 | 206.5 |
| 218 | 112.1 | 261 | 135.1 | 304 | 158.7 | 347 | 182.6 | 390 | 207.1 |
| 219 | 112.7 | 262 | 135.7 | 305 | 159.3 | 348 | 183.2 | 391 | 207.7 |
| 220 | 113.2 | 263 | 136.2 | 306 | 159.8 | 349 | 183.7 | 392 | 208.3 |
| 221 | 113.7 | 264 | 136.8 | 307 | 160.4 | 350 | 184.3 | 393 | 208.8 |
| 222 | 114.3 | 265 | 137.3 | 308 | 160.9 | 351 | 184.9 | 394 | 209.4 |
| 223 | 114.8 | 266 | 137.8 | 309 | 161.5 | 352 | 185.4 | 395 | 210.0 |
| 224 | 115.3 | 267 | 138.4 | 310 | 162.0 | 353 | 186.0 | 396 | 210.6 |
| 225 | 115.9 | 268 | 138.9 | 311 | 162.6 | 354 | 186.6 | 397 | 211.2 |
| 226 | 116.4 | 269 | 139.5 | 312 | 163.1 | 355 | 187.2 | 398 | 211.7 |
| 227 | 116.9 | 270 | 140.0 | 313 | 163.7 | 356 | 187.7 | 399 | 212.3 |
| 228 | 117.4 | 271 | 140.6 | 314 | 164.2 | 357 | 188.3 | 400 | 212.9 |
| 229 | 118.0 | 272 | 141.1 | 315 | 164.8 | 358 | 188.9 | 401 | 213.5 |
| 230 | 118.5 | 273 | 141.7 | 316 | 165.3 | 359 | 189.4 | 402 | 214.1 |
| 231 | 119.0 | 274 | 142.2 | 317 | 165.9 | 360 | 190.0 | 403 | 214.6 |
| 232 | 119.6 | 275 | 142.8 | 318 | 166.4 | 361 | 190.6 | 404 | 215.2 |
| |
| (A) | (B) | (A) | (B) | (A) | (B) | (A) | (B) | (A) | (B) |
| 405 | 215.8 | 417 | 222.8 | 429 | 229.8 | 441 | 236.9 | 453 | 244.0 |
| 406 | 216.4 | 418 | 223.3 | 430 | 230.4 | 442 | 237.5 | 454 | 244.6 |
| 407 | 217.0 | 419 | 223.9 | 431 | 231.0 | 443 | 238.1 | 455 | 245.2 |
| 408 | 217.5 | 420 | 224.5 | 432 | 231.6 | 444 | 238.7 | 456 | 245.7 |
| 409 | 218.1 | 421 | 225.1 | 433 | 232.2 | 445 | 239.3 | 457 | 246.3 |
| 410 | 218.7 | 422 | 225.7 | 434 | 232.8 | 446 | 239.8 | 458 | 246.9 |
| 411 | 219.3 | 423 | 226.3 | 435 | 233.4 | 447 | 240.4 | 459 | 247.5 |
| 412 | 219.9 | 424 | 226.9 | 436 | 233.9 | 448 | 241.0 | 460 | 248.1 |
| 413 | 220.4 | 425 | 227.5 | 437 | 234.5 | 449 | 241.6 | 461 | 248.7 |
| 414 | 221.0 | 426 | 228.0 | 438 | 235.1 | 450 | 242.2 | 462 | 249.3 |
| 415 | 221.6 | 427 | 228.6 | 439 | 235.7 | 451 | 242.8 | 463 | 249.9 |
| 416 | 222.2 | 428 | 229.2 | 440 | 236.3 | 452 | 243.4 | | |
141. Meissl’s Table for Invert Sugar.—Invert sugar is usually the product of the hydrolysis of sucrose. The following table is to be used when the hydrolysis is complete, i. e., when no sucrose is left in the solution. The solution of copper sulfate and of the alkaline tartrate are made up as follows: 34.64 grams of copper sulfate in half a liter, and 173 grams of rochelle salt and 51.6 grams sodium hydroxid in the same volume. The quantity of sugar solution used must not contain more than 245 nor less than ninety milligrams of invert sugar.
In the determination twenty-five cubic centimeters of the copper solution and an equal volume of the alkaline tartrate are mixed and boiled, the proper amount of sugar solution added to secure a quantity of invertose within the limits named, the volume completed to 100 cubic centimeters with boiling water, and the mixture kept in lively ebullition for two minutes. An equal volume of recently boiled cold water is added and the cuprous oxid at once separated by filtration on asbestos under pressure, and washed free of alkali with boiling water. The metallic copper is secured by one of the methods already described.
Table for Invert Sugar by Meissl and Wien.[107]
- (A) = Milligrams of copper.
- (B) = Milligrams of invert sugar.