Delafield’s Hæmatoxylin.—Dissolve hæmatoxylin, 4 Gm., in absolute alcohol, 25 C.c., and add the solution to 400 C.c. of a saturated aqueous solution of ammonia alum. Expose the mixture to light and air for 3 or 4 days, then filter and add glycerine, 100 C.c., and methylic alcohol, 100 C.c. Again expose the solution to light until it becomes dark-coloured, then filter and preserve in a stoppered bottle.
Ehrlich’s Acid Hæmatoxylin.—Dissolve hæmatoxylin, 2 Gm., in absolute alcohol, 100 C.c., and add glycerine, 100 C.c., distilled water, 100 C.c., ammonia alum, 2 Gm., glacial acetic acid, 10 C.c. Expose to daylight for at least a month before use, removing the stopper at intervals.
Ehrlich’s Hæmatoxylin (Ammoniated).—Dissolve ammonium carbonate, 0·4 Gm., and hæmatoxylin, 2 Gm., in proof spirit, 40 C.c., and expose to the air in a shallow dish for 24 hours. Then make up the volume to 40 C.c. with proof spirit (warming if necessary to re-dissolve any separate crystals), and add ammonia alum, 2 Gm., dissolved in distilled water, 80 C.c., together with glycerine, 100 C.c., rectified spirit, 80 C.c., and glacial acetic acid, 10 C.c.
Ehrlich-Biondi Mixture (or Ehrlich-Biondi-Heidenheim mixture).—Dissolve (a) methyl green, 0·5 Gm., in distilled water, 100 C.c.; (b) acid fuchsine, 0·5 Gm., in distilled water, 40 C.c.; (c) orange, 2 Gm., in distilled water, 200 C.c. Mix the three solutions and filter before use. Stain sections for 12 hours, then wash, dehydrate, clear, and mount.
Ehrlich-Weigert-Koch’s Gentian-Violet-Aniline-Water.—Aniline water, 100 C.c., concentrated alcoholic solution of gentian violet, 11 C.c.; absolute alcohol, 10 C.c.
Everard, Demoor, and Massart’s Hæmatoxylin-Eosine.—Dissolve alum, 20 Gm., in water, 200 Gm., by the aid of heat, then filter, and after 24 hours add a solution of hæmatoxylin, 1 Gm., in alcohol, 10 Gm. Let the solution stand for 8 days, again filter, and mix with an equal volume of the following solution:—Eosine, 1 Gm., alcohol, 25 Gm., water, 75 Gm., glycerine, 50 Gm.
Flemming’s Gentian Violet Method.—Use a concentrated alcoholic solution of Gentian Violet diluted with about one half its bulk of water. Differentiate the stained objects in alcohol acidulated with about 0·5 per cent. of hydrochloric acid, followed by pure alcohol and clove oil.
Flemming’s Orange Method.—Stain for days or weeks in strong alcoholic safranine solution diluted with half its bulk of aniline water (saturated); then rinse in distilled water, differentiate in absolute alcohol containing 0·1 per cent. of hydrochloric acid, stain for 1 to 3 hours in strong aqueous gentian violet solution, again wash in distilled water, and finally treat with concentrated aqueous solution of Orange. After a few minutes transfer sections to absolute alcohol, then clear in clove or bergamot oil, and mount in dammar or balsam.
Fol’s Ferric Chloride Fixing and Staining Process.—Preparations are treated with tincture of ferric chloride diluted with 5 to 10 times its bulk of 70 per cent. alcohol, and then transfer for 24 hours to alcohol containing a trace of gallic acid.
Frey’s Fuchsine Solution.—A solution of 0·01 Gm. of crystallised fuchsine, 20 to 25 drops absolute alcohol, and 15 C.c. of water.