1535. George Collyer, M.A.
1557. Laurence Vaux, B.D., chaplain to the Bishop of Gloucester.
1558. William Bird, M.A.
1570. Thomas Herle, chaplain to Queen Elizabeth.
1578. John Walton, B.D. He was appointed Bishop of Exeter in 1579.
1579. William Chadderton, D.D., consecrated Bishop of Chester in 1579. Manchester by this time had become part of the new see of Chester, and Chadderton retained his wardenship along with the higher office, but he resigned it when he was translated to the see of Lincoln in 1595.
1595. John Dee, M.A., a layman and a celebrated mathematician, alchemist, astrologer, and necromancer, who professed to see visions in crystal globes, and was much consulted by many, among them by the Queen, to forecast future events, held the office of warden for some years, but retired in 1608, and died in poverty at Mortlake, at the age of 81.
1608. Richard Murray, D.D., Rector of Stopford, and Dean of St. Buryan's in Cornwall.
1636. Richard Heyrick, M.A. He was expelled in 1646, but reinstated in his office in 1660. His memorial tablet may be seen on the wall of the south aisle, dated 1667.