case has a sufficient number of characters been studied to show whether there is any correspondence between the number of hereditary groups of characters and the number of chromosomes. In the fruit fly, Drosophila ampelophila, we have found about 125 characters that are inherited in a perfectly definite way. On the opposite page is a list of some of them.

It will be observed in this list that the characters are arranged in four groups, Groups I, II, III and IV. Three of these groups are equally large or nearly so; Group IV contains only two characters. The characters are put into these groups because in heredity the members of each group tend to be inherited together, i.e., if two or more enter the cross together they tend to remain together through subsequent generations. On the other hand, any member of one group is inherited entirely independently of any member of the other groups; in the same way as Mendel's yellow-green pair of characters is inherited independently of the round-wrinkled pair.

Group I
Abnormal
Bar
Bifid
Bow
Cherry
Chrome
Cleft
Club
Depressed
Dot
Eosin
Facet
Forked
Furrowed
Fused
Green
Jaunty
Lemon
Lethals, 13
Miniature
Notch
Reduplicated
Ruby
Rudimentary
Sable
Shifted
Short
Skee
Spoon
Spot
Tan
Truncate intensifier
Vermilion
White
Yellow
Group II
Antlered
Apterous
Arc
Balloon
Black
Blistered
Comma
Confluent
Cream II
Curved
Dachs
Extra vein
Fringed
Jaunty
Limited
Little crossover
Morula
Olive
Plexus
Purple
Speck
Strap
Streak
Trefoil
Truncate
Vestigial
Group III
Band
Beaded
Cream III
Deformed
Dwarf
Ebony
Giant
Kidney
Low crossing over
Maroon
Peach
Pink
Rough
Safranin
Sepia
Sooty
Spineless
Spread
Trident
Truncate intensifier
Whitehead
White ocelli
Group IV
Bent
Eyeless

If the factors for these characters are carried by the chromosomes, then we should expect that those factors that are carried by the same chromosome would be inherited together, provided the chromosomes are definite structures in the cell.

Fig. 52. Chromosomes (diploid) of D. ampelophila. The sex chromosomes are XX in the female and XY in the male. There are three other pairs of chromosomes.

In the chromosome group of Drosophila, (fig. 52) there are four pairs of chromosomes, three of nearly the same size and one much smaller. Not only is there agreement between the number of hereditary groups and the number of the chromosomes, but even the size relations are the same, for there are three great groups of characters and three pairs of large chromosomes, and one small group of characters and one pair of small chromosomes.