Let it be said at once that these results do not offer an altogether satisfactory basis for an answer to the question proposed above. Some of the animals would take up a position during the first ten minutes and remain in it for the rest of the hour. Whether the position taken was due solely to the light, or was owing to thigmotactic influences, or whether it depended on the way in which the animal was released, are questions which cannot be answered, and for this reason the conclusions to be drawn from the table are tentative. If we examine the table we find that the totals of both groups agree in manifesting a decrease in negative results for line II, after exposure to the light, as compared with line I, after confinement in the dark. This is what we would expect from negatively phototactic animals. When taken from the dark the pigment is retracted, and the sensitive retinal substance is exposed to the direct action of a rather strong light. The negative tendency of the animal we should expect to find accentuated. The decrease in negative reactions is especially marked with group 2, which was shown above to be much the livelier of the two, and all the individuals except no. 27 share in the change. In group 1 the decrease is not so striking, and is observed to be due to two individuals solely. Inexplicable is the preponderance of positive over negative reactions in the results for group 1.
All of the experiments thus far described were carried out in water at approximately 15° C. The question naturally arises, what will be the result of raising or lowering the temperature upon the reactions of the animals to light? Unfortunately the experiments anent this question are fragmentary and incomplete, but the results will be given for what they are worth. The same apparatus and the same intensity of light (64 c.) were used as in the preceding paragraph. The results, presented in Table V, are arranged in three sets, as follows: The line marked I represents the results obtained from group 1 at a temperature of 5° C. The animals were placed in the box one at a time, as in Table I, and their orientation noted. They were set at right angles to the rays of light, five times with the right and five with the left side toward the source of the stimulus. No observations were made upon group 2 at 5° C., and those on group 1 are so few as to have a questionable value. Line II gives the reactions of both groups of animals in water at 25° C., and in this set the animals were placed in all four of the positions indicated for Table III. Line III presents the reactions of the animals in water at 25° C. by the method outlined for Table IV, i. e., each group of animals was placed in the centre of the box, and observed at intervals of one minute. To obviate the objection of the animals remaining in one spot, they were reset every ten minutes in the middle of the box, at right angles to the entering rays.
TABLE V. REACTIONS TO LIGHT AT DIFFERENT TEMPERATURES
| Group 1 | Nos. 13 | 33 | 4 | 42 | 9 | Totals | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| + | – | + | – | + | – | + | – | + | – | + | – | |||||||
| I | 7 | 3 | 4 | 6 | 2 | 8 | 2 | 8 | 8 | 2 | 23 | 27 | ||||||
| II | 16 | 4 | 14 | 6 | 11 | 9 | 9 | 11 | 13 | 4 | (±3) | 63 | 34 | (±3) | ||||
| III | 19 | 41 | 13 | 47 | 39 | 21 | 17 | 43 | 27 | 33 | 115 | 185 | ||||||
| Group 2 | Nos. 21 | 27 | 31 | 36 | 38 | Totals | ||||||||||||
| + | – | + | – | + | – | + | – | + | – | + | – | |||||||
| II | 12 | 8 | 5 | 15 | 12 | 8 | 15 | 5 | 13 | 7 | 57 | 43 | ||||||
| III | 12 | 48 | 12 | 48 | 31 | 29 | 42 | 18 | 44 | 16 | 141 | 159 | ||||||
Judging from lines II and III we may say that there is a tendency toward a decrease of the negative phototaxis with an increase in temperature. It is true that group 1 in line III maintains the average, 62% negative reactions, but the others are much lower than this, line II even going over to positive phototaxis in both groups. In line III the animals of both groups were extremely active during the first ten minutes, rushing about from one end of the box to the other, pushing each other back and forth, and in general exhibiting great restlessness. Some of the animals when first put into the water reacted with a sort of cramp reflex, which was followed in a few seconds by intense activity. After the first ten minutes the animals began to grow more quiet, and in twenty or thirty minutes they had become quite sluggish, scarcely moving out of the position in which they were reset. During the period of restlessness the males showed marked sexual activity, rushing up to the females, pushing them about, seizing them, and trying to turn them over in spite of their vigorous resistance. One of the males, no. 36, did succeed in turning a female on her back twice, although she struggled violently to escape,—a thing which the female never does in the ordinary sexual act. The rise in temperature, therefore, seemed to stimulate the males to sexual activity, but not the females.
2. Reactions to Colored Light
No observations have ever been made, so far as I know, on the reactions of the crayfish to colored light. Lyon, in his work on compensatory eye-movements, found that rotation in blue light gave a compensatory movement only slightly less than that in white light, while in red light the compensation was only a little larger than in darkness. In some animals the interposition of an opaque object between the eye and the source of light caused an elevation of the eye 1° or 2° toward the vertical. Red glass acted like an opaque object, blue glass produced no effect, i. e., blue light had the same effect as white light. To observe whether the same thing applied to movement reactions was the object of the following experiments.
a. Reactions to Horizontal Colored Light. The same apparatus was used as in the previous experiments, viz., the dark box with light from the 64 c. lamp entering horizontally at the end. Across half of this end were placed pieces of colored glass of a saturated blue, green, yellow, and red. The colored light obtained by this means was not spectrally pure, but it was the nearest to it that could be obtained. A more serious objection is that the intensities were not the same, the red and the yellow being very appreciably brighter than the blue and the green. In addition to observations with these colors, a piece of black cardboard was introduced in the same position as the glass, thus cutting off the light from that half of the box. This, to preserve the uniformity of the series, is denominated black. The animals were placed in the centre of the box, on the line separating the white from the colored light, and were observed at intervals of one minute for forty minutes, the position of each animal being accurately noted. At the end of every ten minutes the animals were reset at the centre of the box. The following table gives a summary of the results for each individual. Here again it was impossible to keep the groups constant owing to the death of individuals during the progress of the experiment.