Group 1 Group 2
Animals 133741424433494346 Sum 212731363852 Sum Sum Total
Blue 129213018 90 232241337 99 189
White 2831191022 110 173816273 101 211
Green 15 9 233617 100 121932840 102 202
White 25 31 17423 100 28213712 98 198
Yellow 2010312819 108 1711283735 128 236
White 203091221 92 23291235 72 164
Red 26 9 322017 104 230162936 113 217
White 14 31 82023 96 381024114 87 183
Black 2427 12 2726 116 1518 26255 89 205
White 1613 28 1314 84 2522 141535 111 195

In this table the colored lights are arranged in the order of the spectrum from blue to red. On the hypothesis that blue light has practically the same effect upon animal reactions as white light, while red is about the same as darkness, we might expect that the reactions would be about equally divided between the blue and the white, and that there would be a gradually increasing difference in number as we go down the table, reaching the maximum with the last pair, black-white. This, we see, however, is not quite the case. In both groups the white has a slightly larger number of reactions than the blue, while the pair green-white shows numbers more nearly equal. In the sum totals the yellow shows a greater preponderance over the white than any other color, and the black and white are very nearly equal. Group 1, it is true, shows a fairly regular ascending scale in reactions to the colored lights with the exception of the red, and the same might be said of group 2 if it were not for the very low number of reactions to the black and the exceptionally high showing of the yellow. On the whole, however, the differences are so small and the individual variations are so large that we can only conclude that for these conditions colored light has little or no effect on the reactions of the animals.

In the foregoing experiment the light came from a broad spiral coil inside the bulb of the lamp, and the distance from it to the edge of the glass was so small compared with the length of the box that there was no sharp dividing-line between the colored light and the white, but rather a wedge-shaped block of lessening saturation of the color, and this wedge, having the point toward the light, took up the whole of the box at the extreme farther end. Thus the imaginary central line dividing the white light from the colored departed farther and farther from the reality as the rear of the box was approached. To obviate this difficulty and to get a check on the previous work, the following series of experiments was undertaken.

b. Reactions to Vertical Colored Light. The same box was used as in the previous experiments, but the end was closed with a black cloth, and an electric light marked 32 c. but measuring only 22 c. was hung exactly over the middle of the box, 40 cm. from the bottom. By means of wires it was arranged that a plate of colored glass could be swung in such a manner that all of one half the box (the whole of one end) was illuminated with the desired color, while the other half was either left white or illuminated with another color. In this way there was a fairly sharp dividing-line between the two colors. The animals were observed at intervals of one minute for 40 minutes, and reset at the middle on the dividing-line every ten minutes as before. Table VII gives the results of the observations.

In this set of experiments it was possible to keep the groups intact except that in group 1 no. 13 had to be replaced by no. 46. If now we conceive the colors arranged in the order of the spectrum with black at one end and white at the other, and consider the black a lower stimulus than the white, we have the ascending series black, red, yellow, green, blue, white. Now since the animals have already been shown to be somewhat negatively phototactic, we should expect them to prefer a color of lower stimulus to one of higher. Turning to the sum totals in the table we find that the first color of each pair (which is always the lower stimulus) has the larger number of reactions in every case but one, the first pair of red-blue. As was stated above, it was impossible to secure colored light of the same intensity by means of the glass at our disposal, and in the present case the red was considerably brighter than the blue. Owing to the fact already mentioned that different intensities of white light seem to have no effect on the reactions it was thought that these differences in the intensities of the colored lights might be overlooked. Since the only thing that could be thought of to account for the anomalous behavior to the red-blue was this difference in intensity, another experiment was undertaken with the same animals under slightly different conditions. A glass aquarium about 40 cm. long by 20 cm. wide was covered with black cardboard and black cloth in such a manner that light could enter only through a space 5 cm. wide at the bottom of each end. Each of these ends was covered, the one with blue, the other with red glass, and 15 cm. from each end was placed an electric light marked 32 c. Later, however, it was found that one of these lamps measured 30 c. and the other 22 c. The red light was found to be much more intense to the eye than the blue, so the former was damped down with tissue paper until the two appeared to have the same intensity. The second pair of red-blue in Table VII gives the results of the observations under these conditions, and these are found to be in harmony with the rest of the table, i. e.,[236] the color giving the lower stimulus has the higher number of reactions.

TABLE VII. REACTIONS TO VERTICAL COLORED LIGHT

Group 1 Group 2 Group 3
Animal no.1337414344 Sum 2127363852 Sum 5456586062 SumSum Total
Blue1722142529 107 1121262726 111 2632262810 122340
White2318261511 93 2919141314 89 148141230 78260
Green1726112129 104 212730726 111 293126109 105320
White2314291911 96 1913103314 89 119143031 95280
Yellow2521122035 103 1220342529 120 192092822 98321
White1519282015 97 282061511 80 2120311218 102279
Red3733232228 143 2130303322 136 3237263328 156435
White37171812 57 191010718 64 8314712 44165
Black232012834 106 912254032 112 2532183416 125343
White172039126 94 312815 14 88 15822624 75257
Black2015132737 112 1611162724 94 3640182929 152358
Red202527133 88 2429241316 106 4 221111 48242
Black20[236]25262032 123 2215394032 148 3322343411 134405
Blue201514208 77 18251 8 52 7186629 66195
Red251728188 96 315212820 87 2113151428 91274
Blue1523122232 104 3725191220 113 1927252612 109316
Red[237]2127221036 116 1340242128 126 2225182116 102344
Blue191318304 84 27 161912 74 1815221924 98256

The most striking feature of the table is the marked predominance of the red over the white. Here the red reaches 73% of the total number of reactions, and inspection shows that this predominance is uniform not only through the groups but even for the individuals. The constancy of this reaction and the fact that it is so much more frequent than the one to the black as compared with the white, would lead one to expect that the red would have the higher percentage in the combination black-red. Such, however, is not found to be the case, although it does happen with one group. If the arrangement of our color-scale in accordance with increasing intensity of stimulus were correct, we should expect a gradually increasing predominance in reactions to colored light over those to white in the first five pairs. Instead of this we find that green and yellow stand nearest to the white, blue and black come next and are almost equal, while red is very much higher than any. In the pairs black-red and black-blue the red holds its predominance over the blue at about the same rate as in the second pair of the direct comparison, red-blue. The wide individual variations, however, in all these reactions to colored light, except perhaps in the case of red-white, indicate that there is nothing very regular, stereotyped, or mechanical about them. The most that can be said is that in a general way the red end of the spectrum furnishes a less intense stimulus to negative reaction than the blue.

A tendency to habit formation was noticed during the course of these experiments, and it is possible that this may have influenced the results somewhat. Many individuals apparently formed a habit of going to a certain corner as soon as they were reset at the centre. The positions in which they were set were varied and they were headed in different directions, but within a minute after they were released in the middle of the box they would be found in their favorite corner. This was especially the case with no. 38 in Table VI, and I think accounts in some measure for the persistent avoidance of the white. In no case did this continue throughout the whole series, but would sometimes be noted for two or three days at a time in the case of an individual. What were the controlling factors in this habit formation, the means by which orientation and recognition were effected, I was unable to determine.