CHEMICAL NATURE OF STARCH GRAINS.
Dr. Brukner has contributed to the Proceedings of the Vienna Academy of Sciences a paper on the "Chemical Nature of the Different Varieties of Starch," especially in reference to the question whether the granulose of Nageli, the soluble starch of Jessen, the amylodextrin of W. Nageli, and the amidulin of Nasse are the same or different substances. A single experiment will serve to show that under certain conditions a soluble substance maybe obtained from starch grains.
If dried starch grains are rubbed between two glass plates, the grains will be seen under the microscope to be fissured, and if then wetted and filtered, the filtrate will be a perfectly clear liquid showing a strong starch reaction with iodine. Since no solution is obtained from uninjured grains, even after soaking for weeks in water, Brukner concludes that the outer layers of the starch grains form a membrane protecting the interior soluble layers from the action of the water.
The soluble filtrate from starch paste also contains a substance identical with granulose. Between the two kinds of starch, the granular and that contained in paste, there is no chemical but only a physical difference, depending on the condition of aggregation of their micellæ.
W. Nageli maintains that granulose, or soluble starch, differs from amylodextrin in the former being precipitated by tannic acid and acetate of lead, while the latter is not. Brukner fails to confirm this difference, obtaining a voluminous precipitate with tannic acid and acetate of lead in the case of both substances. Another difference maintained by Nageli, that freshly precipitated starch is insoluble, amylodextrin soluble in water, is also contested; the author finding that granulose is soluble to a considerable extent in water, not only immediately after precipitation, but when it has remained for twenty-four hours under absolute alcohol. Other differences pointed out by W. Nageli, Brukner also maintains to be non-existent, and he regards amidulin and amylodextrin as identical. Brucke gave the name erythrogranulose to a substance nearly related to granulose, but with a stronger affinity for iodine, and receiving from it not a blue but a red color. Brukner regards the red color as resulting from a mixture of erythrodextrin, and the greater solubility of this substance in water.
If a mixture of filtered potato starch paste and erythrodextrin is dried in a watch glass covered with a thin pellicle of collodion, and a drop of iodine solution placed on the latter, it penetrates very slowly through the pellicle, the dextrin becoming first tinctured with red, and the granulose afterward with blue. If, on the other hand, no erythrodextrin is used, the diffusion of the iodine causes at once simply a blue coloring.
With regard to the iodine reaction of starch, Brukner contests Sachsse's view as to the loss of color of iodide of starch at a high temperature. He shows that the iodide may resist heat, and that the loss of color depends on the greater attraction of water for iodine as compared with starch, and the greater solubility of iodine in water at high temperatures.
The different kinds of starch do not take the same tint with the same quantity of (solid) iodine. That from the potato arum gives a blue, and that from wheat and rice a violet tint; while the filtrate from starch paste, from whatever source, always gives a blue color.