Theoretically, 100 parts of sugar should give 51 parts of alcohol and 49 parts of carbon dioxide gas. This figure has been shown by Browne to be a little high. In actual practice, 45-47 parts of alcohol from 100 parts of sugar is a fair average.
But why not add "mother" or vinegar to sweet cider or put [Page 11] sweet cider into an old vinegar barrel? Here is the reason: We have seen from what has gone before that alcohol is produced from the fermentation of the sugar. We shall soon learn that the acetic acid of the vinegar is formed from this alcohol. Now, in order to obtain the maximum amount of acetic acid, it is necessary to have as much alcohol as possible in the hard cider, and this can be obtained only by the complete conversion of all the sugar into alcohol and carbon dioxide gas. The complete destruction of the sugar can be accomplished only by the uninterrupted action of the yeast, and the presence of "mother" of vinegar by producing acetic acid interferes seriously with this fermentation. The yeast cells are either killed or their useful activity is checked long before all of the sugar has been changed into alcohol. This is the condition of a very large percentage of farm vinegar—just hard cider that will not and never will make vinegar. It means just this:
A small part of the sugar was made into alcohol and this alcohol was at once changed to acetic acid by the "mother" present; this acetic acid killed the yeast so that no more sugar could be changed to alcohol and no more alcohol being found, no more acetic acid could be made by the "mother." We have a weak, worthless something neither vinegar nor hard cider with considerable unfermented sugar still present and incapable of further fermentation because no yeast can develop in the weak acetic acid solution.
The Acetic Acid Fermentation.
The second step in vinegar making is the change of the alcohol of the hard cider into the acetic acid of the finished product. This is accomplished by the acetic acid germ, another microscopic plant still smaller than the yeast. In some peculiar way it is able to bring about a union between the alcohol of the hard cider and the oxygen of the air so that the alcohol is transformed into acetic acid and water.
As soon as the alcoholic fermentation, described in the preceding section, is completed, draw off the clear liquid, being very careful not to disturb the sediment in the barrel. Wash out the barrel thoroughly and replace the hard cider. It is believed that removing this sediment permits the acetic acid to form somewhat more quickly, and furthermore, the sediment may undergo decomposition and impart a disagreeable flavor to the cider. Again, these dregs may harbor living bacteria which either destroy acetic acid or interfere with its formation.
This done, we are now ready to introduce the acetic acid [Page 12] germs. This may be carried out in a number of different ways, but preferably by means of a pure culture of a desirable organism which has been selected because of its ability to produce strong acetic acid and to impart an agreeable flavor to the vinegar. In place of the pure culture starter, one may add two to four quarts of good cider vinegar containing more or less "mother" for each barrel. The introduction of a desirable organism is left to chance in this case. A serious objection to this latter method is that sometimes one introduces foreign organisms with the "mother" which may prove detrimental to the vinegar. The pure culture starter is free from this objection. On the whole, the indiscriminate use of "mother" alone is to be discouraged, since the popular idea of what constitutes "mother" is apt to be wrong. Pure "mother" is made up exclusively of acetic acid bacteria and is recognized as the thin, white, glistening, gelatinous membrane that forms on the surface of vinegar. It seldom becomes one sixteenth of an inch in thickness and should be translucent or white in color, It is entirely distinct from the thick, tough, dark brown, slipping, leathery masses which form in vinegar and are usually regarded as "mothy of vinegar." Such accumulations contain the acetic acid germ, in all probability, but in an impure state. In addition to this organism there may be present yeast cells and numerous bacteria which are positively harmful to the vinegar. Often these growths undergo decomposition and give the vinegar a flavor of rotten oranges. Again the germs present may cause the partial or complete loss of the acid, particularly if the barrels are not full and tightly stoppered. All things taken into consideration, the use of this sort of "mother" is a rather dangerous procedure.
With the acetic fermentation, as with the alcoholic, the higher temperatures favor the changes. Experimental work shows that hard cider to which no acetic acid bacteria were added other than those that came from the air, and kept at 65 degrees F., when six months old, contained 7.03 per cent. of acetic acid, while that held at 55 degrees F. showed only 3.63 per cent.
The addition of some kind of an acetic acid starter, either as a pure culture of the acetic organism or as good vinegar, hastens the fermentation and reduces appreciably the time required for making marketable vinegar.
For most satisfactory results we would recommend using the pure cultures and holding the vinegar at a temperature of 65 to 75 degrees F. Under these conditions, salable vinegar can be obtained in three to six months in place of two to three years, as is often [Page 13] the case. Theoretically, 100 parts of alcohol should give about 130 parts of acetic acid, but in actual practice this will probably fall below 120.