May 4: 10 a. m., about 10 cc thick, dark-colored mucilaginous urine found in collecting bottle; albumin a little more than a trace, decidedly less than on May 3, no sugar, condition of dog pretty good except for slight muscular tremors; 4 p. m., 2 grams caffein by mouth (as usual).
May 5: Urine not examined, no symptoms; 4 p. m., 2 grams caffein.
May 6: Urine not examined; 2.30 p. m., 2.5 grams caffein given by mouth; 4 p. m., slight tremor, no other symptoms.
May 7: No examination of urine, no symptoms observed; 4 p. m., 2.5 grams caffein.
May 8: 9 a. m., found dead, urine collected since last dose of caffein was given did not contain any sugar or albumin, the amount of caffein fed to this dog was 18 grams in 18 days. Autopsy: Post-mortem examination showed marked enteritis with hemorrhagic spots on the mucosa; liver and kidneys congested and dark colored; lungs congested; thyroid gland was greatly enlarged and congested.
Dog 28.
April 30: Weight, 6.8 kilos; the diet consisted of 250 grams rice, 250 grams sugar, 100 grams cracker meal, and 100 grams of meat. On May 3 his weight was 7 kilos. He received 1 gram of caffein by mouth at 12 noon. At 2 p. m. he vomited and tremors were observed. The next day, May 4, tremors were still present though less pronounced. Examination of the urine for sugar and albumin was negative; on May 4, 1 gram caffein was given again and repeated on May 5. On this date his general condition was not good—dog had no appetite and refused to take caffein. As the dog lost 10 per cent of his weight he was put on a meat diet exclusively and the dose of caffein was reduced to 0.5 gram. He became sick after the second dose, and the administration of caffein was therefore discontinued. It was resumed after five days and the caffein was administered in increasing amounts, i. e., on May 18, 0.5 gram; May 19, 0.5; May 20, 1; May 21, 1 gram in two doses of 0.5 each, given at intervals of one hour; May 22, 1 gram. Dog became irritable, but no other symptoms were observed. The administration of caffein was omitted the next day. On the following day when the same dose of caffein was given there was again marked irritability and tremors. The experiment was therefore discontinued.
Dog 22. Male bulldog.
June 24: Dog weighed 13.7 kilos. Diet consisted of meat exclusively; 1 gram caffein was given by mouth; diarrhea developed; no caffein was given for three days.
June 28: Dog weighed 13.6 kilos, 1.5 grams caffein given at 10 a. m.